on the other hand, various methods of isolation of spermatids from testis are available 另一方面,已经报道了多种不同的方法可从睾丸中分离精子细胞。
this method of isolation, cultivation and purification of the synovial cells in vitro is proved to be simple and feasible 结论本研究建立了简单易行的滑膜细胞分离、培养和纯化方法。
to explore the methods of isolation and purification of human fetal liver stem cells in vitro and analyze the bionomics 摘要研究人胎肝干细胞体外分离纯化方法,并对其进行初步鉴定及生物学特性分析。
occurs as a white or nearly white, granular or crystalline powder and as a mixture of various states of hydration, up to the trihydrate, depending on the method of isolation 本品为白色结晶性或颗粒性粉末本品在沸水中极易溶解,在水中溶解,在无水乙醇氯仿或乙醚中不溶。
thus, in order to investigate the developmental pathways not only involved in the regulation of growth and patterning, but also in the determination of cell lineages and differentiation, we utilized the fluorescent immunohistochemical methods, flow cytometry analysis sorting ( facs ) and molecular methods to investigate the developmental law of mammary gland at the different developmental stages, distribution of the stem cells in mammary gland, the methods of isolation, culture and evaluation for the stem cells, the multipotent abilities in vivo and in vitro, and the efficient cultural system for stem cells enriched in vitro . the results showed below : 1 我们以小鼠为模型,运用组织化学、免疫荧光组织(细胞)化学、流式细胞仪分选方法(facs)以及分子生物学手段,研究了小鼠乳腺的发育规律:小鼠乳腺组织中类乳腺干细胞:小鼠乳腺细胞的分离、培养以及类乳腺干细胞的鉴定;小鼠类乳腺干细胞分化的潜能;小鼠乳腺类腺体体外短期培养富集类乳腺干细胞体系的优化等。研究结果表明:1